>UpCell®
Cornea
Cardiac myoblast
Chondrocyte
Esophagus cancer
Hepatocyte
Keratinocyte
Lung cell
MDCK cell
Mesenchymal stem cell
Mesothelial cell
Microglia
Periodontal ligament
Retinal pigment cell
Skeletal myocyte
Smooth muscle cell
Urothelial cell
Suface modification
Review

>RepCell®

>HydroCell®

>AquaWay®

 ■UpCell® references/publications
■Cornea
Watanabe, K., Yamato, M., Hayashida, Y., Yang, J., Kikuchi, A., Okano, T., Tano, Y., and Nishida, K. (2007). Development of transplantable genetically modified corneal epithelial cell sheets for gene therapy. Biomaterials 28, 745-749.
Obokata, H., Yamato, M., Yang, J., Nishida, K., Tsuneda, S., and Okano, T. (2007). Subcutaneous transplantation of autologous oral mucosal epithelial cell sheets fabricated on temperature-responsive culture dishes. Journal of biomedical materials research. Part A
Kanayama, S., Nishida, K., Yamato, M., Hayashi, R., Sugiyama, H., Soma, T., Maeda, N., Okano, T., and Tano, Y. (2007). Analysis of angiogenesis induced by cultured corneal and oral mucosal epithelial cell sheets in vitro. Experimental eye research 85, 772-781.
Sumide, T., Nishida, K., Yamato, M., Ide, T., Hayashida, Y., Watanabe, K., Yang, J., Kohno, C., Kikuchi, A., Maeda, N., Watanabe, H., Okano, T. and Tano, Y. (2006). Functional human corneal endothelial cell sheets harvested from temperature-responsive culture surfaces. The FASEB Journal 20, 392-394.
Ide, T., Nishida, K., Yamato, M., Sumide, T., Utsumi, M., Nozaki, T., Kikuchi, A., Okano, T., and Tano, Y. (2006). Structural characterization of bioengineered human corneal endothelial cell sheets fabricated on temperature-responsive culture dishes. Biomaterials 27, 607-614.
Hayashida, Y., Nishida, K., Yamato, M., Yang, J., Sugiyama, H., Watanabe, K., Hori, Y., Maeda, N., Kikuchi, A., Okano, T. and Tano, Y. (2006). Transplantation of tissue-engineered epithelial cell sheets after excimer laser photoablation reduces postoperative corneal haze. Investigative ophthalmology & visual science 47, 552-557.
Umemoto, T., Yamato, M., Nishida, K., Kohno, C., Yang, J., Tano, Y., and Okano, T. (2005). Rat limbal epithelial side population cells exhibit a distinct expression of stem cell markers that are lacking in side population cells from the central cornea. FEBS letters 579, 6569-6574.
Hayashida, Y., Nishida, K., Yamato, M., Watanabe, K., Maeda, N., Watanabe, H., Kikuchi, A., Okano, T., and Tano, Y. (2005). Ocular surface reconstruction using autologous rabbit oral mucosal epithelial sheets fabricated ex vivo on a temperature-responsive culture surface. Investigative ophthalmology & visual science 46, 1632-1639.
Watanabe, K., Nishida, K., Yamato, M., Umemoto, T., Sumide, T., Yamamoto, K., Maeda, N., Watanabe, H., Okano, T., and Tano, Y. (2004). Human limbal epithelium contains side population cells expressing the ATP-binding cassette transporter ABCG2. FEBS letters 565, 6-10.
Nishida, K., Yamato, M., Hayashida, Y., Watanabe, K., Yamamoto, K., Adachi, E., Nagai, S., Kikuchi, A., Maeda, N., Watanabe, H., Okano, T. and Tano, Y. (2004). Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium. The New England journal of medicine 351, 1187-1196.
Nishida, K., Yamato, M., Hayashida, Y., Watanabe, K., Maeda, N., Watanabe, H., Yamamoto, K., Nagai, S., Kikuchi, A., Tano, Y. and Okano, T. (2004). Functional bioengineered corneal epithelial sheet grafts from corneal stem cells expanded ex vivo on a temperature-responsive cell culture surface. Transplantation 77, 379-385.

■Cardiac myocyte
Masuda, S., Shimizu, T., Yamato, M., and Okano, T. (2008). Cell sheet engineering for heart tissue repair. Advanced drug delivery reviews 60, 277-285.
Shimizu, T., Sekine, H., Yang, J., Isoi, Y., Yamato, M., Kikuchi, A., Kobayashi, E., and Okano, T. (2006). Polysurgery of cell sheet grafts overcomes diffusion limits to produce thick, vascularized myocardial tissues. The FASEB journal 20, 708-710.
Shimizu, T., Sekine, H., Isoi, Y., Yamato, M., Kikuchi, A., and Okano, T. (2006). Long-term survival and growth of pulsatile myocardial tissue grafts engineered by the layering of cardiomyocyte sheets. Tissue engineering 12, 499-507.
Sekine, H., Shimizu, T., Yang, J., Kobayashi, E., and Okano, T. (2006). Pulsatile myocardial tubes fabricated with cell sheet engineering. Circulation 114, I87-93.
Sekiya, S., Shimizu, T., Yamato, M., Kikuchi, A., and Okano, T. (2006). Bioengineered cardiac cell sheet grafts have intrinsic angiogenic potential. Biochemical and biophysical research communications 341, 573-582.
Sekine, H., Shimizu, T., Kosaka, S., Kobayashi, E., and Okano, T. (2006). Cardiomyocyte bridging between hearts and bioengineered myocardial tissues with mesenchymal transition of mesothelial cells. J Heart Lung Transplant 25, 324-332.
Haraguchi, Y., Shimizu, T., Yamato, M., Kikuchi, A., and Okano, T. (2006). Electrical coupling of cardiomyocyte sheets occurs rapidly via functional gap junction formation. Biomaterials 27, 4765-4774.
Furuta, A., Miyoshi, S., Itabashi, Y., Shimizu, T., Kira, S., Hayakawa, K., Nishiyama, N., Tanimoto, K., Hagiwara, Y., Satoh, T., Fukada, K., Okano, T. and Ogawa, S. (2006). Pulsatile cardiac tissue grafts using a novel three-dimensional cell sheet manipulation technique functionally integrates with the host heart, in vivo. Circulation research 98, 705-712.
Itabashi, Y., Miyoshi, S., Yuasa, S., Fujita, J., Shimizu, T., Okano, T., Fukuda, K., and Ogawa, S. (2005). Analysis of the electrophysiological properties and arrhythmias in directly contacted skeletal and cardiac muscle cell sheets. Cardiovascular research 67, 561-570.
Shimizu, T., Yamato, M., Kikuchi, A., and Okano, T. (2003). Cell sheet engineering for myocardial tissue reconstruction. Biomaterials 24, 2309-2316.
Shimizu, T., Yamato, M., Isoi, Y., Akutsu, T., Setomaru, T., Abe, K., Kikuchi, A., Umezu, M., and Okano, T. (2002). Fabrication of pulsatile cardiac tissue grafts using a novel 3-dimensional cell sheet manipulation technique and temperature-responsive cell culture surfaces. Circulation research 90, e40-e48.
Shimizu, T., Yamato, M., Akutsu, T., Shibata, T., Isoi, Y., Kikuchi, A., Umezu, M., and Okano, T. (2002). Electrically communicating three-dimensional cardiac tissue mimic fabricated by layered cultured cardiomyocyte sheets. Journal of biomedical materials research 60, 110-117.
Shimizu, T., Yamato, M., Kikuchi, A., and Okano, T. (2001). Two-dimensional manipulation of cardiac myocyte sheets utilizing temperature-responsive culture dishes augments the pulsatile amplitude. Tissue Engineering 7, 141-151.
Miyagawa, S., Sawa, Y., Sakakida, S., Taketani, S., Kondoh, H., Memon, I.A., Imanishi, Y., Shimizu, T., Okano, T., and Matsuda, H. (2005). Tissue cardiomyoplasty using bioengineered contractile cardiomyocyte sheets to repair damaged myocardium: their integration with recipient myocardium. Transplantation 80, 1586-1595.

■Chondrocyte
Kaneshiro, N., Sato, M., Ishihara, M., Mitani, G., Sakai, H., and Mochida, J. (2006). Bioengineered chondrocyte sheets may be potentially useful for the treatment of partial thickness defects of articular cartilage. Biochemical and biophysical research communications 349, 723-731.

■Esophagus cancer
Ohki, T., Yamato, M., Murakami, D., Takagi, R., Yang, J., Namiki, H., Okano, T., and Takasaki, K. (2006). Treatment of oesophageal ulcerations using endoscopic transplantation of tissue-engineered autologous oral mucosal epithelial cell sheets in a canine model. Gut 55, 1704-1710.
Murakami, D., Yamato, M., Nishida, K., Ohki, T., Takagi, R., Yang, J., Namiki, H., and Okano, T. (2006). The effect of micropores in the surface of temperature-responsive culture inserts on the fabrication of transplantable canine oral mucosal epithelial cell sheets. Biomaterials 27, 5518-5523.
Murakami, D., Yamato, M., Nishida, K., Ohki, T., Takagi, R., Yang, J., Namiki, H., and Okano, T. (2006). Fabrication of transplantable human oral mucosal epithelial cell sheets using temperature-responsive culture inserts without feeder layer cells. Journal of artificial organs 9, 185-191.

■Hepatocyte
Takayama, G., Taniguchi, A., and Okano, T. (2007). Identification of differentially expressed genes in hepatocyte/endothelial cell co-culture system. Tissue engineering 13, 159-166.
Ohashi, K., Yokoyama, T., Yamato, M., Kuge, H., Kanehiro, H., Tsutsumi, M., Amanuma, T., Iwata, H., Yang, J., Okano, T. and Nakajima, Y. (2007). Engineering functional two- and three-dimensional liver systems in vivo using hepatic tissue sheets. Nat Med 13, 880-885.
Kurosawa, Y., Taniguchi, A., and Okano, T. (2005). Novel method to examine hepatocyte-specific gene expression in a functional coculture system. Tissue engineering 11, 1650-1657.
Harimoto, M., Yamato, M., Hirose, M., Takahashi, C., Isoi, Y., Kikuchi, A., and Okano, T. (2002). Novel approach for achieving double-layered cell sheets co-culture: overlaying endothelial cell sheets onto monolayer hepatocytes utilizing temperature-responsive culture dishes. Journal of biomedical materials research 62, 464-470.
Hirose, M., Yamato, M., Kwon, O.H., Harimoto, M., Kushida, A., Shimizu, T., Kikuchi, A., and Okano, T. (2000). Temperature-responsive surface for novel co-culture systems of hepatocytes with endothelial cells: 2-D patterned and double layered co-cultures. Yonsei medical journal 41, 803-813.

■Keratinocyte
Yamato, M., Utsumi, M., Kushida, A., Konno, C., Kikuchi, A., and Okano, T. (2001). Thermo-responsive culture dishes allow the intact harvest of multilayered keratinocyte sheets without dispase by reducing temperature. Tissue engineering 7, 473-480.

■Lung cell
Kanzaki, M., Yamato, M., Yang, J., Sekine, H., Kohno, C., Takagi, R., Hatakeyama, H., Isaka, T., Okano, T., and Onuki, T. (2007). Dynamic sealing of lung air leaks by the transplantation of tissue engineered cell sheets. Biomaterials 28, 4294-4302.
Kanzaki, M., Yamato, M., Hatakeyama, H., Kohno, C., Yang, J., Umemoto, T., Kikuchi, A., Okano, T., and Onuki, T. (2006). Tissue engineered epithelial cell sheets for the creation of a bioartificial trachea. Tissue engineering 12, 1275-1283.
Nandkumar, M.A., Yamato, M., Kushida, A., Konno, C., Hirose, M., Kikuchi, A., and Okano, T. (2002). Two-dimensional cell sheet manipulation of heterotypically co-cultured lung cells utilizing temperature-responsive culture dishes results in long-term maintenance of differentiated epithelial cell functions. Biomaterials 23, 1121-1130.

■MDCK cell
Kushida, A., Yamato, M., Isoi, Y., Kikuchi, A., and Okano, T. (2005). A noninvasive transfer system for polarized renal tubule epithelial cell sheets using temperature-responsive culture dishes. European cells & materials 10, 23-30; discussion 23-30.
Kushida, A., Yamato, M., Kikuchi, A., and Okano, T. (2001). Two-dimensional manipulation of differentiated Madin-Darby canine kidney (MDCK) cell sheets: the noninvasive harvest from temperature-responsive culture dishes and transfer to other surfaces. Journal of biomedical materials research 54, 37-46.
Kushida, A., Yamato, M., Konno, C., Kikuchi, A., Sakurai, Y., and Okano, T. (2000). Temperature-responsive culture dishes allow nonenzymatic harvest of differentiated Madin-Darby canine kidney (MDCK) cell sheets. Journal of biomedical materials research 51, 216-223.

■Mesenchymal stem cell
Miyahara, Y., Nagaya, N., Kataoka, M., Yanagawa, B., Tanaka, K., Hao, H., Ishino, K., Ishida, H., Shimizu, T., Kangawa, K., Sano, S., Okano, T., Kitamura, S. and Mori, H. (2006). Monolayered mesenchymal stem cells repair scarred myocardium after myocardial infarction. Nature medicine 12, 459-465.

■Mesothelial cell
Asano, T., Takazawa, R., Yamato, M., Takagi, R., Iimura, Y., Masuda, H., Kihara, K., and Okano, T. (2006). Transplantation of an autologous mesothelial cell sheet prepared from tunica vaginalis prevents post-operative adhesions in a canine model. Tissue engineering 12, 2629-2637.
Asano, T., Takazawa, R., Yamato, M., Kageyama, Y., Kihara, K., and Okano, T. (2005). Novel and simple method for isolating autologous mesothelial cells from the tunica vaginalis. BJU international 96, 1409-1413.

■Microglia
Nakajima, K., Honda, S., Nakamura, Y., Lopez-Redondo, F., Kohsaka, S., Yamato, M., Kikuchi, A., and Okano, T. (2001). Intact microglia are cultured and non-invasively harvested without pathological activation using a novel cultured cell recovery method. Biomaterials 22, 1213-1223.

■Periodontal ligament
Gomez Flores, M., Hasegawa, M., Yamato, M., Takagi, R., Okano, T., and Ishikawa, I. (2008). Cementum-periodontal ligament complex regeneration using the cell sheet technique. Journal of periodontal research
Hasegawa, M., Yamato, M., Kikuchi, A., Okano, T., and Ishikawa, I. (2005). Human periodontal ligament cell sheets can regenerate periodontal ligament tissue in an athymic rat model. Tissue engineering 11, 469-478.
Akizuki, T., Oda, S., Komaki, M., Tsuchioka, H., Kawakatsu, N., Kikuchi, A., Yamato, M., Okano, T., and Ishikawa, I. (2005). Application of periodontal ligament cell sheet for periodontal regeneration: a pilot study in beagle dogs. Journal of periodontal research 40, 245-251.

■Retinal pigment epithelial cell
Kubota, A., Nishida, K., Yamato, M., Yang, J., Kikuchi, A., Okano, T., and Tano, Y. (2006). Transplantable retinal pigment epithelial cell sheets for tissue engineering. Biomaterials 27, 3639-3644.
Abe, T., Hojo, M., Saigo, Y., Yamato, M., Okano, T., Wakusawa, R., and Tamai, M. (2006). Retinal pigment epithelial cells from thermally responsive polymer-grafted surface reduce apoptosis. Advances in experimental medicine and biology 572, 363-366.

■Skeletal myoblast
Kondoh, H., Sawa, Y., Miyagawa, S., Sakakida-Kitagawa, S., Memon, I.A., Kawaguchi, N., Matsuura, N., Shimizu, T., Okano, T., and Matsuda, H. (2006). Longer preservation of cardiac performance by sheet-shaped myoblast implantation in dilated cardiomyopathic hamsters. Cardiovascular research 69, 466-475.
Hata, H., Matsumiya, G., Miyagawa, S., Kondoh, H., Kawaguchi, N., Matsuura, N., Shimizu, T., Okano, T., Matsuda, H., and Sawa, Y. (2006). Grafted skeletal myoblast sheets attenuate myocardial remodeling in pacing-induced canine heart failure model. The Journal of thoracic and cardiovascular surgery 132, 918-924.
Memon, I.A., Sawa, Y., Fukushima, N., Matsumiya, G., Miyagawa, S., Taketani, S., Sakakida, S.K., Kondoh, H., Aleshin, A.N., Shimizu, T., Okano, T. and Matsuda, H. (2005). Repair of impaired myocardium by means of implantation of engineered autologous myoblast sheets. The Journal of thoracic and cardiovascular surgery 130, 1333-1341.

■Smooth muscle cell
Hobo, K., Shimizu, T., Sekine, H., Shin'oka, T., Okano, T., and Kurosawa, H. (2008). Therapeutic Angiogenesis Using Tissue Engineered Human Smooth Muscle Cell Sheets. Arteriosclerosis, thrombosis, and vascular biology 28, 637-643

■Urothelial cell
Shiroyanagi, Y., Yamato, M., Yamazaki, Y., Toma, H., and Okano, T. (2004). Urothelium regeneration using viable cultured urothelial cell sheets grafted on demucosalized gastric flaps. BJU international 93, 1069-1075.
Shiroyanagi, Y., Yamato, M., Yamazaki, Y., Toma, H., and Okano, T. (2003). Transplantable urothelial cell sheets harvested noninvasively from temperature-responsive culture surfaces by reducing temperature. Tissue engineering 9, 1005-1012.

■Surface modification
Nishi, M., Kobayashi, J., Pechmann, S., Yamato, M., Akiyama, Y., Kikuchi, A., Uchida, K., Textor, M., Yajima, H., and Okano, T. (2007). The use of biotin-avidin binding to facilitate biomodification of thermoresponsive culture surfaces. Biomaterials 28, 5471-5476.
Tsuda, Y., Shimizu, T., Yamato, M., Kikuchi, A., Sasagawa, T., Sekiya, S., Kobayashi, J., Chen, G., and Okano, T. (2007). Cellular control of tissue architectures using a three-dimensional tissue fabrication technique. Biomaterials 28, 4939-4946.
Hatakeyama, H., Kikuchi, A., Yamato, M., and Okano, T. (2006). Bio-functionalized thermoresponsive interfaces facilitating cell adhesion and proliferation. Biomaterials 27, 5069-5078.
Hatakeyama, H., Kikuchi, A., Yamato, M., and Okano, T. (2005). Influence of insulin immobilization to thermoresponsive culture surfaces on cell proliferation and thermally induced cell detachment. Biomaterials 26, 5167-5176.
Ebara, M., Yamato, M., Aoyagi, T., Kikuchi, A., Sakai, K., and Okano, T. (2004). Immobilization of cell-adhesive peptides to temperature-responsive surfaces facilitates both serum-free cell adhesion and noninvasive cell harvest. Tissue engineering 10, 1125-1135.
Ebara, M., Yamato, M., Aoyagi, T., Kikuchi, A., Sakai, K., and Okano, T. (2004). Temperature-responsive cell culture surfaces enable "on-off" affinity control between cell integrins and RGDS ligands. Biomacromolecules 5, 505-510.
Ebara, M., Yamato, M., Hirose, M., Aoyagi, T., Kikuchi, A., Sakai, K., and Okano, T. (2003). Copolymerization of 2-carboxyisopropylacrylamide with N-isopropylacrylamide accelerates cell detachment from grafted surfaces by reducing temperature. Biomacromolecules 4, 344-349.
Kwon, O.H., Kikuchi, A., Yamato, M., and Okano, T. (2003). Accelerated cell sheet recovery by co-grafting of PEG with PIPAAm onto porous cell culture membranes. Biomaterials 24, 1223-1232.
Kwon, O.H., Kikuchi, A., Yamato, M., Sakurai, Y., and Okano, T. (2000). Rapid cell sheet detachment from poly(N-isopropylacrylamide)-grafted porous cell culture membranes. Journal of biomedical materials research 50, 82-89.
Yamato, M., Konno, C., Kushida, A., Hirose, M., Utsumi, M., Kikuchi, A., and Okano, T. (2000). Release of adsorbed fibronectin from temperature-responsive culture surfaces requires cellular activity. Biomaterials 21, 981-986.
Uchida, K., Sakai, K., Ito, E., Kwon, O.H., Kikuchi, A., Yamato, M., and Okano, T. (2000). Temperature-dependent modulation of blood platelet movement and morphology on poly(N-isopropylacrylamide)-grafted surfaces. Biomaterials 21, 923-929.

■Review
Yang, J., Yamato, M., Shimizu, T., Sekine, H., Ohashi, K., Kanzaki, M., Ohki, T., Nishida, K., and Okano, T. (2007). Reconstruction of functional tissues with cell sheet engineering. Biomaterials 28, 5033-5043.
Yang, J., Yamato, M., Nishida, K., Ohki, T., Kanzaki, M., Sekine, H., Shimizu, T., and Okano, T. (2006). Cell delivery in regenerative medicine: the cell sheet engineering approach. Journal of controlled release 116, 193-203.
Yang, J., Yamato, M., Nishida, K., Hayashida, Y., Shimizu, T., Kikuchi, A., Tano, Y., and Okano, T. (2006). Corneal epithelial stem cell delivery using cell sheet engineering: not lost in transplantation. Journal of drug targeting 14, 471-482.

■RepCell® references/publications

McNulty, A.K., Schmidt, M., Feeley, T., and Kieswetter, K. (2007). Effects of negative pressure wound therapy on fibroblast viability, chemotactic signaling, and proliferation in a provisional wound (fibrin) matrix. Wound Repair Regen 15, 838-846.
Yanase, Y., Suzuki, H., Tsutsui, T., Uechi, I., Hiragun, T., Mihara, S., and Hide, M. (2007). Living cell positioning on the surface of gold film for SPR analysis. Biosensors & bioelectronics 23, 562-567.
Butts, C.L., Shukair, S.A., Duncan, K.M., Harris, C.W., Belyavskaya, E., and Sternberg, E.M. (2007). Effects of dexamethasone on rat dendritic cell function. Hormone and metabolic research Hormon- und Stoffwechselforschung 39, 404-412.
Butts, C.L., Shukair, S.A., Duncan, K.M., Bowers, E., Horn, C., Belyavskaya, E., Tonelli, L., and Sternberg, E.M. (2007). Progesterone inhibits mature rat dendritic cells in a receptor-mediated fashion. International immunology 19, 287-296.
Alabraba, E.B., Curbishley, S.M., Lai, W.K., Wigmore, S.J., Adams, D.H., and Afford, S.C. (2007). A new approach to isolation and culture of human Kupffer cells. J Immunol Methods 326, 139-144.
Gordon, I.O., and Freedman, R.S. (2006). Defective antitumor function of monocyte-derived macrophages from epithelial ovarian cancer patients. Clin Cancer Res 12, 1515-1524.

■HydroCell® references/publications

Sasaki, R., Aoki, S., Yamato, M., Uchiyama, H., Wada, K., Okano, T., and Ogiuchi, H. (2008). Neurosphere generation from dental pulp of adult rat incisor. The European journal of neuroscience 27, 538-548.
Yanase, Y., Suzuki, H., Tsutsui, T., Uechi, I., Hiragun, T., Mihara, S., and Hide, M. (2007). Living cell positioning on the surface of gold film for SPR analysis. Biosensors & bioelectronics 23, 562-567.

■AquaWay® references/publications
Kobayashi J., Kikuchi A., Sakai K., and Okano T. Aqueous Chromatography Utilizing pH-/Temperature-Responsive Polymer Stationary Phases To Separate Ionic Bioactive Compounds. Anal. Chem. 73, 2027-2033 (2001)
Kanazawa H., Sunamoto T., Matsushima Y., Kikuchi A., and Okano T. Temperature-Responsive Chromatographic Separation of Amino Acid Phenylthiohydantoins Using Aqueous Media as the Mobile Phase. Anal. Chem. 72, 5961-5966 (2000)
Kanazawa H., Matsushima Y., and Okano T. Temperature-responsive chromatography. Trends in analytical chemistry. 17, 435-440 (1998)