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- Aqua Way (Aqua Way Philic)
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Application data of Aqua Way HPLC column Series
This page highlights the application data on Aqua Way Philic and Aqua Way Cation.
- Direct analysis of human serum with durability data by column pressure and peak area (Aqua Way Philic and Aqua Way Cation )
- Separations of small molecules under 100% aqueous conditions (Aqua Way Philic )
- Separation of proteins (chymotrypsinogen A and albumin) under aqueous solution (Aqua Way Cation and Aqua Way Cation )
- Separation of nucleotide (AMP, ADP and ATP) (Aqua Way Cation )
- Separation of NADP+ / NADPH (Aqua Way Cation )
1. Direct analysis of human serum (Aqua Way Philic and Aqua Way Cation ).
Direct analysis of human serum
- The PIPAAm grafted temperature-responsive surface of the beads is designed to release serum at molecule releasing temperature.
- Therefore direct analysis of bioactive molecules within the serum is possible.
- By optimizing the column temperature for such analysis, calibration curve for drug concentration can be calculated.
- Particularly suitable for therapeutic drug monitoring where no known antibodies to be used for ELIZA are available such as leads compounds.
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| Sample: Carbamizepine in serum (Aqua Way Philic ) |
Sample: Theophylline in human serum (Aqua Way Philic ) |
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| Sample: Theophylline in human serum (Aqua Way Cation ) |
Sample: Primidone in human serum (Aqua Way Philic ) |
Remarkable durability! No cloggings observed beyond 100th analysis!
- The surface of the beads is highly resistent to the adherence of the component of the serum, thus achieving high durability.
- No change in the peak area or the peak shape observed beyond 100th analysis of serum. The constant column pressure also backs up the durability data of the column.
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| (Aqua Way Philic ) | (Aqua Way Philic ) |
2. Separation of small molecules under 100% aqueous condition (Aqua Way Philic ).
- The surface of the beads for Aqua Way Philic changes its hydrophobicity depending on the temperature.
- Lower the temperature, the higher the hydrophilicity of the beads' surface, leading to shorter retention time.
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| Samples: Hydrocortisone, cortisone, prednisolone, prednisolone acetate, testosterone (Aqua Way Philic ) |
3. Separation of proteins (chymotrypsinogen A and albumin) under 100% aqueous solution
(Aqua Way Philic and Aqua Way Cation ).
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| Sample: Chymotrypsinogen A (Aqua Way Philic ) |
- Suitable for separating hydrophobic proteins with small molecular weight of less than MW25,000.
- Samples that can not be separated at 40℃ may be analyzable by using temperature-gradient similar to that of conventional elution gradient.
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| Sample: Albumin (Aqua Way Cation ) |
- By optimizing the elution condition, larger molecules such as albumin can also be separeted.
4. Separation of anionic molecules (AMP, ADP and ATP) (Aqua Way Cation ).
- The strength of the cationic charge of the beads can be controlled by controlling column temperature.
- Higher the temperature, the longer the retention time as the surface charge.
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| Sample: Albumin (Aqua Way Cation ) |
5. Separation of NADP+/NADPH (Aqua Way Cation )
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| Sample: NADP+ / NADPH (Aqua Way Cation ) |