Labware

Advantages

Advantages of UpCell

Image of the Cell-sheet harvest

• The cells produce their own Extra Cellular Matrix (ECM) to initially bind to the surface of the UpCell , followed by cell proliferation.
• When cells are cultured beyond over confluence, it can be harvested as a "cell-sheet" by mere regulation of external temperature instead of cell damaging enzymes such as trypsin or dispase. "Cell-sheet" is a contiguous cells in a form of thin film, not isolated cells and free from polymer contamination.
• So-harvested Cell-sheet retains intact extra cellular matrix (ECM) thus suture is not required when transplanting to the host. The ECM acts as a biological binding glue thus accelerate the cell-sheet adhesion without the need for the suture.
• Since the cell surface recepters and antigens are also kept intact due to cell friendly temperature harvest, simple layering of cell-sheets leads to construction of 3D tissue. Cell-sheets communicate and connect to each other through the channels expressed on the surface. The vertical gap junction is formed as short as 30 minutes (results may vary by cell types and specific culture conditions).

Advantage of the temperature cell harvest technology