Labware

The protocol for collagen type I coating on the surface of UpCell (For the case of HUVEC cells).

Materials

1. UpCell 3.5cm dish
2. HUVEC cells (Lonza, C2517A), medium (Lonza, EGM-2)
3. Collagen I (3.34mg/mL) (BD Bioscience, 354136)
4. 0.02N Acetic acid solution
5. PBS

Collagen coating protocol

We recommend you to increase the concentration of the material by 5 to 10 times to the normal protocol and coat the UpCell surface with collagen solution and incubate at 37 degrees C.

1. Prepare 500μg/mL of collagen solution by 0.02 Acetic acid solution.
2. Add 1mL of the collagen solution onto the surface of UpCell 3.5cm dish and coat all the entire surface.
3. Incubate for 1 hour at 37 degree C incubator.
4. Remove the collagen solution from UpCell and wash with pre-warmed 1mL of PBS.

Culture protocol

1. Seed HUVEC cells (10,000 cells/cm²) onto collagen coated UpCell 3.5cm dish.
2. Incubate at 37 degree C incubator for over 2 days until cells overconfluence.

Comparison of cells 2 days after seeding

Without coating

Coating with culture medium (Pre-incubation at 37 degrees for 1 hours before seeding)

Coating with collagen