1) Detachment of cells by control of temperature. 2) Comparison of re-attachment kinetics for harvested A-549 cells between RepCell and trypsin. 3) Comparison of harvested extra cellular matrix between RepCell and dispase (E-cadherin). 4) Comparison of flow cytometry application between RepCell and trypsin. 5) Comparison of detachment of macrophages between RepCell and trypsin. 6) Comparison of re-attachment of macrophages between RepCell and trypsin.