Labware

Protocol for dendritic cell collection in single cell suspension.

Cell culture conditions

1. Cell type: KG-1, JCRB9051/ATCC  CCL-246
2. Cell density: 2.0 x 10⁵ cells/UpCell 3.5cm
3. Culture period: 7 days after differentiation induction
4. Culture medium: 10%FBS-RPMI 1640, 10nM PMA, 10ng/mL TNFalfa

* Cell culture condition may vary by cell type.
(JCRB: Japanese Collection of Research Bioresources, ATCC: American Type Culture Collection)

Methodology

1. Seed cells in low density so that cells do not stick to each other (A,B).
2. Leave UpCell at room temperature (20-25℃) for 30 minutes.
3. Slight agitation with pipet will prompt cell detachment (D).
4. Cells do not come off for control dish (C).