Labware

Protocol for macrophage collection in single cell suspension

Cell culture conditions

1. Cell type : HL-60, JCRB0085/ATCC CCL-240
2. Cell density : 1.0 x 10⁵ cells/UpCell 3.5cm
3. Culture period : 2 days after differentiation induction
4. Culture medium : 10%FBS-RPMI 1640

* Cell culture condition may vary by cell type.
(JCRB: Japanese Collection of Research Bioresources, ATCC: American Type Culture Collection)

Methodology

1. Seed cells in low density so that cells do not stick to each other (A,B).
2. Leave UpCell at room temperature (20-25℃) for 15 minutes.
3. Slight agitation with pipet will prompt cell detachment (D).
4. Cells do not come off for control dish (C).