1) Detachment of cells by control of temperature.

2) Comparison of re-attachment kinetics for harvested A-549 cells between UpCell/RepCell (UpCell with Grid) and trypsin.

3) Comparison of harvested extra cellular matrix between UpCell/RepCell (UpCell with Grid) and dispase (E-cadherin).

4) Comparison of flow cytometry application between UpCell/RepCell (UpCell with Grid) and trypsin.

5) Comparison of detachment of macrophage between UpCell/RepCell (UpCell with Grid) and trypsin.

6) Comparison of re-attachment of macrophage between UpCell/RepCell (UpCell with Grid) and trypsin.

7) Protocol for macrophage collection in single cell suspension

8) Protocol for dendritic cell collection in single cell suspension.

9) Protocol of NIH/3T3 cell-sheet transfer using membranes for UpCell 3.5cm.

10) Application data for mono-layered cell-sheet.

11) Application data for multiple layered cell-sheet (3D tissue constructs).

7) Protocol for macrophage collection in single cell suspension

Cell culture conditions 1) Cell type: HL-60, JCRB0085/ATCC CCL-240 2) Cell density: 1.0 x 105 cells/UpCell 3.5cm 3) Culture period: 2 days after differentiation induction 4) Culture medium: 10%FBS-RPMI 1640 * Cell culture condition may vary by cell type. (JCRB: Japanese Collection of Research Bioresources ATCC: American Type Culture Collection) Methodology 1) Seed cells in low density so that cells do not stick to each other (A,B). 2) Leave UpCell at room temperature (20-25C) for 15 minutes. 3) Slight agitation with pipet will prompt cell detachment (D). 4) Cells do not come off for control dish (C). .