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Application Data

Comparison of re-attachment of macrophage between UpCell/RepCell and trypsin.

RepCell EDTA/Scraping
UpCell/RepCell (UpCell with Grid) EDTA/scraping
  1. Seed 5x105 of peritoneal macrophages (mouse) onto RepCell and tissue culture treated dish (6cm).
  2. Incubate for 2 hours, followed by wash off of anchorage independent cells with PBS.
  3. Further culture for 2 days. Replace medium with PBS. Detach cells with temperature (on ice, 5min) for RepCell and PBS+EDTA.
  4. Re-seed detached cells onto tissue culture dishes respectively.
  5. Observe cells after 24 hours of incubation.