1) Detachment of cells by control of temperature.

2) Comparison of re-attachment kinetics for harvested A-549 cells between UpCell/RepCell (UpCell with Grid) and trypsin.

3) Comparison of harvested extra cellular matrix between UpCell/RepCell (UpCell with Grid) and dispase (E-cadherin).

4) Comparison of flow cytometry application between UpCell/RepCell (UpCell with Grid) and trypsin.

5) Comparison of detachment of macrophage between UpCell/RepCell (UpCell with Grid) and trypsin.

6) Comparison of re-attachment of macrophage between UpCell/RepCell (UpCell with Grid) and trypsin.

7) Protocol for macrophage collection in single cell suspension

8) Protocol for dendritic cell collection in single cell suspension.

9) Protocol of NIH/3T3 cell-sheet transfer using membranes for UpCell 3.5cm.

10) Application data for mono-layered cell-sheet.

11) Application data for multiple layered cell-sheet (3D tissue constructs).

6) Comparison of re-attachment of macrophage between UpCell /RepCell and trypsin.

UpCell/RepCell (UpCell with Grid)		EDTA/scraping
1) Seed 5x105 of peritoneal macrophages (mouse) onto RepCell and tissue culture treated dish (6cm). 2) Incubate for 2 hours, followed by wash off of anchorage independent cells with PBS. 3) Further culture for 2 days. Replace medium with PBS. Detach cells with temperature (on ice, 5min) for RepCell and PBS+EDTA. 4) Re-seed detached cells onto tissue culture dishes respectively. 5) Observe cells after 24 hours of incubation.