Labware
- Laboratory consumables and equipments
- UpCell
- RepCell
- HydroCell
- Aqua Way (Aqua Way Philic)
- Aqua Way (Aqua Way Cation)
- Distributors
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Application Data
Comparison of detachment of macrophage between RepCell and trypsin.
| Normal culture @ 37 degrees celsius |
30 min post-cool @ 20 degrees celsius |
Removal after slight agitation |
|
|---|---|---|---|
| Peritoneal macrophage (mouse) |
 |
 |
 |
Recovery ratio of peritoneal macrophages (mouse)
 |
- Seed 5x106 of peritoneal macrophages (mouse) on 1 (one) RepCell and 2 (two) tissue culture treated 6cm dish respectively.
- Incubate for 2 hours, and wash off anchorage-independent cells by PBS.
- Further culture for 2 days. Replace medium with PBS. Detach cell by temperature (on ice, 5min) for RepCell, 2.5ml trypsin and EDTA/scraping for tissue culture dishes, followed by measurement.
Recovery ratio of macrophage (human)
 |
- Seed 5x106 of HL-60 onto RepCell and tissue treated cell culture dish (6cm). Add 100nm PMA (Phorbol 12 Myristate 130 Acetate) to induce cells to macrophages.
- Culture for 5 days and replace medium with PBS. Detach cells by temperature (25℃, 30min) for RepCell and EDTA/PBS for tissue culture treated dish.
(Prof. Kobayashi, Toho Univ. Biosci.)