| Upcell/RepCell (UpCell with Grid) data
5) Comparison of detachment of macrophage between RepCell and trypsin.
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Normal culture
@ 37 degrees celsius |
30 min post-cool
@ 20 degrees celsius |
Removal after
slight agitation |
| Peritoneal macrophage (mouse) |
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Recovery ratio of peritoneal macrophages (mouse)
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1) Seed 5x106 of peritoneal macrophages (mouse) on 1 (one) RepCell and 2 (two) tissue culture treated 6cm dish respectively.
2) Incubate for 2 hours, and wash off anchorage-independent cells by PBS.
3) Further culture for 2 days. Replace medium with PBS. Detach cell by temperature (on ice, 5min) for RepCell, 2.5ml trypsin and EDTA/scraping for tissue culture dishes, followed by measurement.
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Recovery ratio of macrophage (human)
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1) Seed 5x106 of HL-60 onto RepCell and tissue treated cell culture dish (6cm). Add 100nm PMA (Phorbol 12 Myristate 130 Acetate) to induce cells to macrophages.
2) Culture for 5 days and replace medium with PBS. Detach cells by temperature (25°C, 30min) for RepCell and EDTA/PBS for tissue culture treated dish.
(Prof. Kobayashi, Toho Univ. Biosci.)
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