1) Detachment of cells by control of temperature.

2) Comparison of re-attachment kinetics for harvested A-549 cells between UpCell/RepCell (UpCell with Grid) and trypsin.

3) Comparison of harvested extra cellular matrix between UpCell/RepCell (UpCell with Grid) and dispase (E-cadherin).

4) Comparison of flow cytometry application between UpCell/RepCell (UpCell with Grid) and trypsin.

5) Comparison of detachment of macrophage between UpCell/RepCell (UpCell with Grid) and trypsin.

6) Comparison of re-attachment of macrophage between UpCell/RepCell (UpCell with Grid) and trypsin.

7) Protocol for macrophage collection in single cell suspension

8) Protocol for dendritic cell collection in single cell suspension.

9) Protocol of NIH/3T3 cell-sheet transfer using membranes for UpCell 3.5cm.

10) Application data for mono-layered cell-sheet.

11) Application data for multiple layered cell-sheet (3D tissue constructs).

4) Comparison of flow cytometry application between RepCell and trypsin.

	RepCell	Trypsin
Bone marrow cells (human)
Preadipocyte (human)
Methodologies:	1) Seed 6.8 x 103/cm2 cells onto RepCell and tissue culture treated dish. 2) Cells are harvested by temperature reduction for RepCell (20°C, 30min) and trypsin (37°C, 3min). 3) Incubate detached cells with PE antibody for 1 hours at 4°C, followed by analysis with FC500 (Beckman Coulter).
Antibody used:	・CD140a(PDGFRα)-PE:Pharmingen,　556002,αR1, Ab1µg/2.5x105cells/0.2mL 1%FBS-PBS, ・Mouse IgG2a-PE(Isotype control):Pharmingen, 5592529, MPC-11, Ab 1µg/2.5x105 cells/0.2mL 1%FBS-PBS,