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Application Data

Comparison of flow cytometry application between UpCell/RepCell (UpCell with Grid) and trypsin.

  RepCell Trypsin
Bone marrow cells
(human)
Preadipocyte
(human)

Methodologies

  1. Seed 6.8 x 103/cm2 cells onto RepCell and tissue culture treated dish.
  2. Cells are harvested by temperature reduction for RepCell (20℃, 30min) and trypsin (37℃, 3min).
  3. Incubate detached cells with PE antibody for 1 hours at 4℃, followed by analysis with FC500 (Beckman Coulter).

Antibody used

  • CD140a(PDGFRα)-PE:Pharmingen, 556002,αR1, Ab1µg/2.5x105cells/0.2mL 1%FBS-PBS,
  • Mouse IgG2a-PE(Isotype control):Pharmingen, 5592529, MPC-11, Ab 1µg/2.5x105 cells/0.2mL 1%FBS-PBS,