1) Detachment of cells by control of temperature.

2) Comparison of re-attachment kinetics for harvested A-549 cells between UpCell/RepCell (UpCell with Grid) and trypsin.

3) Comparison of harvested extra cellular matrix between UpCell/RepCell (UpCell with Grid) and dispase (E-cadherin).

4) Comparison of flow cytometry application between UpCell/RepCell (UpCell with Grid) and trypsin.

5) Comparison of detachment of macrophage between UpCell/RepCell (UpCell with Grid) and trypsin.

6) Comparison of re-attachment of macrophage between UpCell/RepCell (UpCell with Grid) and trypsin.

7) Protocol for macrophage collection in single cell suspension

8) Protocol for dendritic cell collection in single cell suspension.

9) Protocol of NIH/3T3 cell-sheet transfer using membranes for UpCell 3.5cm.

10) Application data for mono-layered cell-sheet.

11) Application data for multiple layered cell-sheet (3D tissue constructs).

10) Application data for mono-layered cell-sheet.

Autologous cornea epithelium cell-sheet has been transplanted to corneal limbal stem cell deficiency (LSCD) dog model. Reconstruction of the corneal surface has been achieved successfully. (Data kindly provided by Dr. Endoh (DVM), of Kunitachi Animal Hospital) References on cornea epithelium reconstruction: 1) Hayashida, Y., Nishida, K., Yamato, M., Watanabe, K., Maeda, N., Watanabe, H., Kikuchi, A., Okano, T., and Tano, Y. (2005). Ocular surface reconstruction using autologous rabbit oral mucosal epithelial sheets fabricated ex vivo on a temperature-responsive culture surface. Investigative ophthalmology & visual science 46, 1632-1639. 2) Nishida, K., Yamato, M., Hayashida, Y., Watanabe, K., Yamamoto, K., Adachi, E., Nagai, S., Kikuchi, A., Maeda, N., Watanabe, H., Okano, T. and Tano, Y. (2004). Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium. The New England journal of medicine 351, 1187-1196. 3) Nishida, K., Yamato, M., Hayashida, Y., Watanabe, K., Maeda, N., Watanabe, H., Yamamoto, K., Nagai, S., Kikuchi, A., Tano, Y. and Okano, T. (2004). Functional bioengineered corneal epithelial sheet grafts from corneal stem cells expanded ex vivo on a temperature-responsive cell culture surface. Transplantation 77, 379-385. .