Labware

Application data for mono-layered cell-sheet.

Autologous cornea epithelium cell-sheet has been transplanted to corneal limbal stem cell deficiency (LSCD) dog model. Reconstruction of the corneal surface has been achieved successfully. (Data kindly provided by Dr. Endoh (DVM), of Kunitachi Animal Hospital)

References on cornea epithelium reconstruction:

1. Hayashida, Y., Nishida, K., Yamato, M., Watanabe, K., Maeda, N., Watanabe, H., Kikuchi, A., Okano, T., and Tano, Y. (2005). Ocular surface reconstruction using autologous rabbit oral mucosal epithelial sheets fabricated ex vivo on a temperature-responsive culture surface. Investigative ophthalmology & visual science 46, 1632-1639.
2. Nishida, K., Yamato, M., Hayashida, Y., Watanabe, K., Yamamoto, K., Adachi, E., Nagai, S., Kikuchi, A., Maeda, N., Watanabe, H., Okano, T. and Tano, Y. (2004). Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium. The New England journal of medicine 351, 1187-1196.
3. Nishida, K., Yamato, M., Hayashida, Y., Watanabe, K., Maeda, N., Watanabe, H., Yamamoto, K., Nagai, S., Kikuchi, A., Tano, Y. and Okano, T. (2004). Functional bioengineered corneal epithelial sheet grafts from corneal stem cells expanded ex vivo on a temperature-responsive cell culture surface. Transplantation 77, 379-385.