1) Detachment of cells by control of temperature.

2) Comparison of re-attachment kinetics for harvested A-549 cells between UpCell/RepCell (UpCell with Grid) and trypsin.

3) Comparison of harvested extra cellular matrix between UpCell/RepCell (UpCell with Grid) and dispase (E-cadherin).

4) Comparison of flow cytometry application between UpCell/RepCell (UpCell with Grid) and trypsin.

5) Comparison of detachment of macrophage between UpCell/RepCell (UpCell with Grid) and trypsin.

6) Comparison of re-attachment of macrophage between UpCell/RepCell (UpCell with Grid) and trypsin.

7) Protocol for macrophage collection in single cell suspension

8) Protocol for dendritic cell collection in single cell suspension.

9) Protocol of NIH/3T3 cell-sheet transfer using membranes for UpCell 3.5cm.

10) Application data for mono-layered cell-sheet.

11) Application data for multiple layered cell-sheet (3D tissue constructs).

1) Detachment of cells by control of temperature.

	Normal culture @ 37 degrees celsius	30 min post-cool @ 20 degrees celsius	Removal after slight agitation
A549
Swiss　3T3
NRK
Mesenchymal stem cells
Peritoneal macrophage (mice)