Shimizu T., Yamato M., Akutsu T., Shibata T., Isoi Y., Kikuchi A., Umezu M., and Okano T. : Electrically communicating three-dimensional cardiac tissue mimic fabricated by layerd cultured cardiomyocyte sheets. J Biomed Mater. Res. 60,110-117 (2002) Yamato M., Konno C., Utsumi M., Kikuchi A., and Okano T. : Thermally responsive polymer-grafted surfaces facilitate patterned cell seeding and co-culture. Biomaterials 23, 561-567 (2002) Shimizu T., Yamato M., Isoi Y., Akutsu T., Setomaru T., Abe K., Kikuchi A., Umezu M., and Okano T. : Fabrication of Pulsatile Cardiac Tissue Grafts Using a Novel 3-Dimensional Cell Sheet Manipulation Technique and Temperature-Responsive Cell Culture Surfaces. Circ Res. 90, 40-48 (2002) Shimizu T., Yamato M., Kikuchi A., and Okano T.: Two-dimensional manipulation of cardiac myocyte sheets utilizing temperature-responsive culture dishes augments the pulsatile amplitude. Tissue Engineering 7, 141-151 (2001) Yamato M., Utsumi M., Kushida A., Konno C., Kikuchi A., and Okano T. :Thermo-responsive culture dishes allow the intact harvest of multi-layered keratinocyte sheets without dispase by reducing temperature. Tissue Engineering 7, 473-480 (2001) Nakajima K., Honda S., Nakamura Y., Lopez-Redondo F., Kohsaka S., Yamato M., Kikuchi A., and Okano T. Intact microglia are cultured and non-invasively harvested without pathological activation using a novel cultured cell recovery method. Biomaterials 22, 1213-1233 (2001) Kushida A., Yamato M., Konno C. Kikuchi A., and Okano T. Temperature-responsive culture dishes allow non-enzymatic harvest of differentiated Madin-Darby canine kidney(MDCK) cell sheets. J. Biomed. Mater. Res. 51, 216-223 (2000) Kushida A., Yamato M., Konno C. Kikuchi A., and Okano T. Non-enzymatic harvest of renal epithelial cell sheets using temperature-responsive culture dishes. Trans. Materials Res. Soc. Japan 25, 903-906 (2000) Hirose M., Kwon O.H., Yamato M., Kikuchi A., and Okano T. Creation of designed shape cell sheets that are non-invasively harvested and moved onto another surface. Biomacromolecule 1, 377-381 (2000) Yamato M., Konno C., Kushida A., Hirose M., Utsumi M., Kikuchi A., and Okano T. Release of adsorbed fibronectin from temperature-responsive culture surfaces requires cellular activity. Biomaterials 21, 981-986 (2000) Uchida K. U., Sakai K., Kwon O. H., Ito E., Aoyagi T., Kikuchi A., Yamato M., and Okano T. Temperature-sensitive poly(N-isopropylacrylamide)-grafted surfaces modulate blood platelet interactions. Macromol. Rapid Commun. 21, 169-173 (2000) Kwon O. H., Kikuchi A., Yamato M., Sakurai Y., and Okano T. Rapid cell sheet detachment from poly(N-isopropylacrylamide)-grafted porous cell culture membranes. J. Biomed. Mater. Res. 50, 82-89 (2000) Hosoya K., Tetsuka K., Nagase K., Tomi M., Saeki S., Ohtsuki S., Terasaki T., Yanai N., Obinata M., Takanaga H., Kikuchi A., and Okano T. Conditionally Immortalized Brain Capillary Endothelial Cell Lines Established from a Transgenic Mouse Harboring Temperature-Sensitive Simian Virus 40 Large T-Antigen Gene. AAPS Pharmsci. 2, article 27, (2000) Von Recum H. A., Okano T., Kim S. W., and Bernstein P. S. Maintenance of retinoid metabolism in human retinal pigment epithelium cell culture. Exp. Eye Res. 69, 97-107 (1999)

Co-culture plate references/publications

Harimoto M., Yamato M., Hirose M., Takahashi C., Isoi Y., Kikuchi A., Umezu M., and Okano T. Novel approach for achieving double-layered cell sheets co-culture: overlaying endotheliala cell sheets onto monlayer hepatocytes utilizing temperature-responsive culture dishes. J Biomed Mater. Res. 62, 464-470 (2002) Nandkumar M. A., Yamato M., Kushida A., Konno C., Hirose M., Kikuchi A., and Okano T. : Two-dimensional cell sheet manipulation of heterotypically co-cultured lung cells utilizing temperature-responsive culture dishes results in long-term maintenance of differentiated epithelial cell functions. Biomaterils 23, 1121-1130 (2002) Yamato M., Konno C., Utsumi M., Kikuchi A., and Okano T. : Thermally responsive polymer-grafted surfaces facilitate patterned cell seeding and co-culture. Biomaterials 23, 561-567 (2002) Yamato M., Kwon O. H., Hirose M., Kikuchi A., and Okano T. Novel patterned cell co culture utilizing thermally responsive grafted polymer surfaces. J. Boomed. Mater. Res. 55, 137-140 (2001) Uchida K., Yamato M., Ito E., Kwon O. H., Kikuchi A., Sakai K., and Okano T. Two different types of nonthrombogenic surfaces: PEG suppresses platelet adhesion ATP-independently but HEMA-St block copolymer requires ATP consumption of platelets to prevent adhesion. J Biomed Mater Res, 50, 585-590 (2000)

AquaWay^® references/publications

Kobayashi J., Kikuchi A., Sakai K., and Okano T. Aqueous Chromatography Utilizing pH-/Temperature-Responsive Polymer Stationary Phases To Separate Ionic Bioactive Compounds. Anal. Chem. 73, 2027-2033 (2001) Kanazawa H., Sunamoto T., Matsushima Y., Kikuchi A., and Okano T. Temperature-Responsive Chromatographic Separation of Amino Acid Phenylthiohydantoins Using Aqueous Media as the Mobile Phase. Anal. Chem. 72, 5961-5966 (2000) Kanazawa H., Matsushima Y., and Okano T. Temperature-responsive chromatography. Trends in analytical chemistry. 17, 435-440 (1998)