Q. Isn't the price too expensive? The advantage of using our product when compared to non-coated products are as follows: 1) If you detach cells by using trypsin, you would require to change pipett chip to new one so as to alleviate contamination when sucking media, and sucking PBS for the first and second time. This renders its total cost to be more expensive than the price of RepCell. 2) Conventional methodology would require 8 times more of pipetting (sucking media, add PBS for the first time, suck PBS, add PBS for the second time, suck PBS, add trypsin and agitation) than RepCell which only requires reduction of the temperature when collecting cells. This leads to the reduction in time used for cloning cells. You can allocate your precious time for more productive work such as writing publications. We have an internal report that suggests the total time spent on the cell cloning procedures were reduced down to 1/5. 3) Because the total procedure could be cut down to 1/5, you can avoid the risk of cross-contamination derived from fatigue, which will eventually improve the accuracy of cell-cloning procedure. 4) Since you do not need to use trypsin to collect cells, the activity of the cells are maintained.

Q. Does the collected grouped cells become single/independent? The polymer coated surface allows collection of grouped cells without damaging its cell-cell junctions. Therefore the collected cells do not automatically become independent or single. However, those cells that has just been divided sometimes become single/independent by pipett agitation. This occurs because cell-cell junction is not so strong if the cells have just been divided.

Q. Do you need temperature control unit which can be lowered down to 20°C? You can achieve the same result by leaving the plate in refrigerator set at 4°C for 30 minutes.