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FAQ
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RepCell
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| What is the difference between UpCell and RepCell (UpCell with Grid)? |
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| Both products are covalently grafted with temperature-responsive polymer. However only RepCell is equipped with 3mm x 3mm of grid walls on the surface of the culture dishes. Grid walls inhibit cells from crossing over them thus enabling easier collection of cells in a form of single cells or in a form of small colonies. Suitable for preparation of cells for flowcytometry analysis. Beware that RepCell (UpCell with Grid) is not suitable for collecting cell-sheet. |
| Does the collected grouped cells become single cell suspension? |
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| Generally speaking, cells with weak cell-cell junction property can be split easily by gentle media pipetting. Please note that the result may vary by cell type and cell culture conditions. |
| Do you need special incubator capable of setting temperature at 20C? |
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| Temperature can also be controlled by leaving UpCell at room temperature (20-25C). Please note that cell detachment performance is constant below 32C, i.e. hydrophilicity of the surface is constant at temperature below LCST therefore too cold the temperature may deprive of cell ATP metabolism, making cell detachment even more difficult. |
| Can you coat the UpCell surface with gellatin or collagen? |
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| Yes, you can. In so doing, coating procedure should be done at 37C. Please note that growth speed of cells may become slower due to this extra surface treatment. |
| What kind of cells are you targeting? |
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| You can culture almost any type of cells like fibroblast such as NIH/3T3, Swiss/3T3 or 10T1/2, mesenchymal stem cells, myoblast or cancer cells. However cells with very strong adherence property may not be detachable with simple temperature reduction. Simultaneous use of diluted trypsin is highly recommended for such case. |
| What options do you have when cells do not come off? |
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| When cells do not come off spontaneously from UpCell surface by simple temperature reduction, please gently pipett adhered cells with cell culture medium., or gently tap the edge of the dish to prompt cell detachment. |
| Do you need to use RepCell (UpCell with Grid) instead of UpCell if you wish to collect cell in a form of single cell suspension? |
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| By seeding cells in low density, you can still collect intact single cell suspension by using UpCell. However RepCell (UpCell with Grid) is more suitable for such purpose.
Following are the recommended protocols for intact single cell collection. (1) When cell-cell junction is tight/strong. Seed cells whose density is so low that cells do not stick to each other. Incubate and harvest within 24 hours before cells start to proliferate again. (2) When cell-cell junction is fragile/weak. Culture cells upto 70-80% of confluency, and collect cells by temperature reduction. Clumped cells can be made to single isolated cells by slight agitation by media pipetting. |