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FAQ
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UpCell
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| What is the storage condition of UpCell? |
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| Please store the product at room temperature. Please avoid direct exposure to sunlight. |
| Is UpCell suitable for repeated usage? |
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| UpCell is designed for single time use only. |
| What are the tips for using UpCell? |
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| "Immediately after seeding" to "upto 24 hours" (1) Please make sure to use cell culture medium pre-warmed at 37C. (2) Try not to lower incubation temperature lower than 37C. (3) Avoid direct shock to UpCell. (4) As much as possible, leave UpCell undisturbed for the first 24 hours of incubation. "During cell culture beyond 24 hours" Avoid observation under microscope at room temperature for long period of time. "During cell culture beyond 24 hours" Avoid observation under microscope at room temperature for long period of time. "Medium change" Make sure to change cell culture medium that is pre-warmed at 37C. |
| Please advise how to collect cell-sheet? |
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| Please refer to the data page. |
| Please advise the pit falls when collecting cell-sheet? |
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| Please refer to the data page. |
| Is usage of UpCell and the supporting membrane essential when collecting cell-sheet? |
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| It is essential to use UpCell when collecting cells as a "cell-sheet". However you can use hygroscopic PVDF membranes as an alternative to the supporting membranes. Please note that optimum protocol may vary by the type of membranes used. |
| What should be done when cells cannot be collected as a cell-sheet even if UpCell and the supporting membranes are used? |
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| The optimum cell-sheet detachment protocol may vary by (1) cellt ype, and (2) culture condition such as cell density, culture period or culture medium. Some optimization may be required. Please refer to Q4 and Q5 for further information. |
| Do cells detach from UpCell when the dish is left at room temperature? |
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| It may take a while for cells to detach from UpCell surface until it becomes hydrophilic, which occurs at temperature lower than LCST of 32C. Therefore followings should be noted. (1) Please make sure to use pre-warmed culture medium whenever changing the culture medium. (2) Beware of time required to change the culture medium. taking too much time to change the culture medium will lower the temperature below LCST. (3) Avoid direct shock to UpCell. Especially cells that had just started to form cell-cell junction are fragile to external shock and may detach by such shock. (4) Usage of such temperature preserving device as hot plate enables you to work longer at room temperature. |
| How can I purchase temperature-responsive cell culture insert? |
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| The temperature-responsive cell culture insert is still under development. The launch schedule shall be advised on this website when known. |
| How can I cryofracture the cell-sheet? How about making paraffin-embedded section with cell-sheet? |
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| Please refer to the data page for further information. |
| What is the protocol of collagen type I coating for UpCell? |
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| Please refer to the data page for further information. |
| What kind of gel is recommended to practice with when transferring cell-sheet harvested from UpCell? |
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| We recommend you to practice with 15% acrylamide gel of SDS-PAGE that is shown in our HP. We also recommend using 7.5% Gelatin gel. However we don't recomend gel with low stickiness such as Agarose. |